Translational_Unit

Part:BBa_K2003002:Design

Designed by: Ida Isolehto   Group: iGEM16_Uppsala   (2016-09-01)


pBad+RBS+CPF1+Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2673
    Illegal BglII site found at 2865
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

designed to express cpf1.This composite biobrick is made of a pBAD/araC (BBa_I0500), a medium RBS (BBa_0032) our his-tagged CPF1 protein and a double terminator (BBa_0015). The arabinose induced promoter was chosen to avoid expression leakage since expression of endonucleases might lead to cell toxicity. However sequencing readings were fuzzy, thus resequencing prior to use is strongly recommended.


Source

CPF1 comes from IDT. The rest comes from igem plates.

References